HRH1 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit) Immunohistochemistry on Parrafin Sections: 4-6 µg/ml In paraffin embedded Human Colon shows membranous staining. 5 µg/ml In paraffin embedded Human Small Intestine. Western blot: Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C Terminus of the protein sequence according to NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1.
Specificity	Recognizes Histamine H1 receptor (C-term).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	histamine receptor H1
Database Link	Entrez Gene 15465 MouseEntrez Gene 24448 RatEntrez Gene 3269 Human UniProt ID P35367
Background	Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. The histamine H1 receptor (HRH1) is an integral membrane protein and belongs to the family 1 of G protein-coupled receptors. It mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, catecholamine release from adrenal medulla, and mediates neurotransmission in the central nervous system.
Synonyms	HRH1, HR-H1, Histamine H1 receptor
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction

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