AKT1 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA: 1/2000 - 1/10000. Immunofluorescence: 1/100 - 1/1000. Immunohistochemistry on Paraffin Sections: 1/100. Immunoprecipitation. Western Blot: 1/500 - 1/2000.
Reactivity	Chicken, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide -KLH conjugated corresponding to the C-terminus (460-480) of Human, Rat, Mouse and Chicken AKT proteins conjugated to KLH using maleimide.
Specificity	Recognizes AKT (AKT1/AKT2/AKT3). The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.
Formulation	0.02 M potassium phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.09% Sodium Azide State: Serum State: Liquid Ig fraction
Concentration	lot specific
Purification	Delipidation and Defibrination
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	AKT serine/threonine kinase 1
Database Link	Entrez Gene 11651 MouseEntrez Gene 24185 RatEntrez Gene 207 Human UniProt ID P31749
Background	AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis and (ii) promotion of proliferation.
Synonyms	Akt-1, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, PKB gamma, Protein kinase B, C-AKT, PKBG, AKT-2, AKT-3 Review 010
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
Protein Pathways	Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway

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