Frizzled 2 (FZD2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Peptide ELISA: Limit Dlution: 1/64000. Western Blot: 0.03-0.1 µg/ml. Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Immunohistochemistry on Paraffin Sections: 2-5 µg/ml in paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. 2.5 µg/ml in paraffin embedded Human Liver. Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_001457.1.
Specificity	Recognizes FZD2 / Frizzled-2.
Formulation	Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	frizzled class receptor 2
Database Link	Entrez Gene 57265 MouseEntrez Gene 64512 RatEntrez Gene 2535 Human UniProt ID Q14332
Background	FZD2 is a Frizzled Receptor that mediates Wnt signaling, which is required for proper axis development in the embryo. FZD2 is developmentally regulated. Studies of rat FZD2 indicate that the protein signals through a calcium-releasing pathway and that FDZ2 expression modulates protein kinase C (PKC) localization and stimulates PKC activity in vitro. Increased levels of FZD2 mRNA have been observed in primary gastric cancer and in head and neck squamous carcinoma and gastric cancer cell lines. FZD2 expression has been documented in human brain, heart, kidney, lung, colon, ovary and stomach. High levels of expression have been observed in fetal kidney and lung and adult colon and ovary. ESTs have been isolated from human tissue libraries, including cancerous kidney, lung, lymph and testis, and normal blood, cervix, embryo and kidney.
Synonyms	Fz-2, hFz2, FzE2
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway

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