Neutral Protease Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques). Working dilutions: vary widely, but may be between 1/1000 and 1/10000.
Reactivity	Bacillus sp.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Neutral protease isolated and purified from Bacillus polymyxa. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to neutral protease from bacillus polymyxa. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~5.1
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P29148
Synonyms	Bacillolysin, npr
Reference Data

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