Phospholipase D Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or histochemical techniques. Working Dilutions: vary widely, but may be between 1/1000 and 1/20000.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phospholipase D isolated and purified from Cabbage. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity	Biotin-conjugated IgG fraction of polyclonal Rabbit antiserum to Phospholipase D from Cabbage. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized Hyperimmune IgG fraction Molar radio: Biotin/IgG ~5.5
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID O82549
Background	Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.
Synonyms	Phospholipase D alpha 1, Phospholipase D alpha 2, Choline phosphatase 1, Choline phosphatase 2, PLD1, PLD-1, PLD2, PLD-2
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.