PLA2G1B Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). Recommended working dilutions: vary widely, but may be between 1/10000 and 1/100000.
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phospholipase A2 isolated and purified from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase A2 from porcine pancreas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Label: Marker: N-Hydroxysuccinimidobiotin. Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life Molar radio: Biotin/IgG ~6.1
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 445525 Pig UniProt ID P00592
Synonyms	PLA2G1B, PLA2, PLA2A, PPLA2, Phospholipase A2, Group IB phospholipase A2
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.