Papain Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques). Working Dilutions: Non-precipitating antibody-binding techniques: 1/10,000-1/100,000.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Papain isolated and purified from Carica papaya. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Papain from Carica papaya. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~6.0
Reconstitution Method	Restore by adding 1.0 ml sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Database Link	UniProt ID P00784
Background	Papain is a protease derived from the papaya fruit (Carica papaya). It hydrolyses peptides with broad specificity, but shows a preference for large hydrophobic amino acids in the P2 position. It is synthesised as an inactive 40 kDa precursor containing a 107 residue N terminal pro region and a 26 residue signal peptide.
Synonyms	Papaya proteinase I, PPI, Allergen Car p 1
Reference Data

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