AFUA_2G14610 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/20,000.
Reactivity	Aspergillus
Host	Rabbit
Clonality	Polyclonal
Immunogen	Oxalate Decarboxylase isolated and purified from Aspergillus species. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Oxalate Decarboxylase from Aspergillus species. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q4X060
Background	Oxalates in animals including humans originate mostly from the diet, especially through ingestion of leafy vegetables and other plant parts. The catabolic pathway of oxalic acid is present in bacteria, fungi, and plants but not in vertebrates. Therefore, management of oxalate in humans is very important because of the absence of any enzyme system that can degrade oxalate. Excess ingestion of oxalate leads to an acute oxalate toxicity, which can result in a variety of disorders including renal failure and urolithiasis (Curhan 1997).
Synonyms	Oxalate Carboxy-lyase, OXDC
Reference Data

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