glpK Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/10,000-1/50,000.
Reactivity	Bacillus sp.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Glycerokinase isolated and purified from Bacillus stearothermophilus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The antibody recognizes Glycerokinase from Bacillus stearothermophilus. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins State: Aff - Purified State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method	Restore by adding 0.5 ml of sterile distilled water
Concentration	lot specific
Purification	Solid Phase Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Database Link	UniProt ID P18157
Background	Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway.
Synonyms	Glycerokinase, GK, GKD
Reference Data

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