TDH1 Rabbit Polyclonal Antibody
CAT#: AP21309AF-N
TDH1 rabbit polyclonal antibody, Azide Free
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CNY 3,402.00
货期*
5周
规格
Cited in 3 publications. |
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommend Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/10,000. |
Reactivity | Bakers Yeast |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Glyceraldehyde-3-Phosphate Dehydrogenase isolated and purified from Baker's Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The antibody recognizes GAPDH from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified Hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Database Link | |
Background | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
Synonyms | GAPD, CDABP0047 |
Reference Data |
Citations (3)
The use of this Antibodies has been cited in the following citations: |
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Disruption of origin chromatin structure by helicase activation in the absence of DNA replication
,null,
Genes & Development
,PubMed ID 34556529
[TDH1]
|
The Heat Shock Response in Yeast Maintains Protein Homeostasis by Chaperoning and Replenishing Proteins
,Mühlhofer, M;Berchtold, E;Stratil, CG;Csaba, G;Kunold, E;Bach, NC;Sieber, SA;Haslbeck, M;Zimmer, R;Buchner, J;,
Cell Rep
,PubMed ID 31875563
[TDH1]
|
High-throughput screening system for inhibitors of human Heat Shock Factor 2
,Smith LM, Bhattacharya D, Williams DJ, Dixon I, Powell NR, Erkina TY, Erkine AM,
Cell Stress Chaperones
,PubMed ID 26003133
[TDH1]
|
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