G6PD Rabbit Polyclonal Antibody
CAT#: AP21260AF-N
G6PD rabbit polyclonal antibody, Azide Free
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CNY 3,402.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommend Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/10,000-1/70,000. |
Reactivity | Leuconostoc |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Glucose-6-Phosphate Dehydrogenase isolated and purified from Leuconostoc mesenteroides. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | This antibody recognizes Glucose-6-Phosphate Dehydrogenase from Leuconostoc mesenteroides. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
Database Link | |
Background | Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency; one benefit of having G6PD deficiency is that it confers a resistance to malaria. |
Synonyms | Glucose-6-phosphate 1-dehydrogenase, Glucose-6-P-Dehydrogenase |
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