ENO3 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/2,000.
Reactivity	Rabbit
Host	Sheep
Clonality	Polyclonal
Immunogen	Enolase isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The antibody recognizes Enolase from Rabbit muscle The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins State: Aff - Purified State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method	Restore by adding 0.5 ml of sterile distilled water
Concentration	lot specific
Purification	Solid Phase Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Database Link	Entrez Gene 100008769 Rabbit UniProt ID P25704
Synonyms	Muscle-specific enolase, Skeletal muscle enolase, Enolase 3, ENO3, ENOB
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.