CDC25A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/500 - 1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of Cdc25A protein.
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 59 kDa
Gene Name	cell division cycle 25A
Database Link	Entrez Gene 12530 MouseEntrez Gene 171102 RatEntrez Gene 993 Human UniProt ID P30304
Background	The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy terminal sequences.
Synonyms	CDC-25A
Reference Data
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	Cell cycle, Progesterone-mediated oocyte maturation

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