RPS6 pSer235 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Immunofluorescence: 1/50 - 1/200. Western blot: 1/500 - 1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at Ser235.
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 32 kDa
Gene Name	ribosomal protein S6
Database Link	Entrez Gene 20104 MouseEntrez Gene 29304 RatEntrez Gene 6194 Human UniProt ID P62753
Background	The genes encoding for mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional introncontaining gene within their coding regions. The RPS6 gene gives rise to Ribosomal Protein S6 (also designated RPS6). RPS6 is the major substrate of protein kinases in eukaryotic ribosomes. Sequence comparison has identified RPS6 as the equivalent of the Ribosomal Protein S10 from Saccharomyces cerevisiae. The sequence comparison of ribosomal proteins from evolutionarily distant eukaryotes, such as yeast and human, indicates that the structure and probably the function of RPS6 has been highly conserved. RPS6 phosphorylation is stimulated by growth factors, tumor promoting agents and mitogens. It is dephosphorylated at growth arrest.
Synonyms	Phosphoprotein NP33, 40S ribosomal protein S6
Reference Data
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway, Ribosome

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