PPP1R14A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 2-50 of Human CPI-17.
Specificity	This antibody detects endogenous levels of CPI-17 protein. (region surrounding Lys32)
Formulation	Phosphate buffered saline (PBS), pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 22 kDa
Gene Name	protein phosphatase 1 regulatory inhibitor subunit 14A
Database Link	Entrez Gene 68458 MouseEntrez Gene 114004 RatEntrez Gene 94274 Human UniProt ID Q96A00
Background	CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.
Synonyms	Protein phosphatase 1 regulatory subunit 14A, CPI-17, CPI17, PPP1INL
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Vascular smooth muscle contraction

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