DNA Ligase I (LIG1) Rabbit Polyclonal Antibody

CAT#: AP20546PU-S

DNA Ligase I (LIG1) rabbit polyclonal antibody, Aff - Purified



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CNY 800.00


货期*
2周

规格
    • 25 ul

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Specifications

Product Data
Applications IHC, WB
Recommend Dilution Western blot: 1/500 - 1/1000.
Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Specificity This antibody detects endogenous levels of DNA Ligase I protein.
(region surrounding Glu139)
Formulation Phosphate buffered saline (PBS), pH 7.2.
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.05% sodium azide
Concentration 1.0 mg/ml
Purification Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size 102,125 kDa
Gene Name DNA ligase 1
Background Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
Synonyms LIG1, LIG-1, DNA ligase 1, EC=6.5.1.1, DNA ligase I
Reference Data
Protein Families Druggable Genome
Protein Pathways Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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