HSP27 (HSPB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide selected from the Center region of human HSP27
Specificity	This antibody reacts to HSPB1 / HSP27.
Formulation	PBS with 0.09% (W/V) sodium azide State: Liquid purified Ig
Concentration	lot specific
Purification	Saturated Ammonium Sulfate (SAS) precipitation
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein family B (small) member 1
Database Link	Entrez Gene 3315 Human UniProt ID P04792
Background	In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
Synonyms	Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Reference Data
Protein Pathways	MAPK signaling pathway, VEGF signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.