AKAP9 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	Peptide ELISA: 1/64000 (Detection Limit). Western Blot: 1-3 µg/ml. This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells.
Reactivity	Human, Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-SGSTTQFHAGMR, from the C-Terminus of the protein sequence according to NP_005742.4; NP_671714.1.
Specificity	This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	A-kinase anchoring protein 9
Database Link	Entrez Gene 10142 Human UniProt ID Q99996
Background	AKAP9 is a novel cytoskeletal protein specifically concentrated in the neuromuscular junction and neuronal synapes that interacts with specific splice variants of NMDA Receptor Subunit NR1 in a C1 exon cassette dependent manner. It has also been shown to physically attach the type I protein phosphatase (PP1) and the adenosine 3',5'-mono-phosphate (cAMP) dependent protein kinase (PKA) holoenzyme to NMDA receptors to regulate channel activity. NMDA receptors are involved with many important functions and dysfunctions of the nervous system, including synapse formation, synaptic plasticity, and excitotoxicity. AKAP9 research may lead to a clearer understanding of these neural processes by explaining how NMDA receptors are targeted to particular synapses, how this localization is regulated during development and synaptic activity, and how NMDA receptor activity is transduced into intracellular signals responsible for particular neuronal responses.
Synonyms	AKAP350, AKAP450, KIAA0803, Protein hyperion, Protein yotiao, AKAP120-like protein
Reference Data

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