SOCS4 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection limit). Western blot: Preliminary experiments in Human and Rodent Brain lysates and in lysates of cell lines NIH3T3, A431 and HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. In paraffin embedded Human Pancreas shows cytoplasm staining on select beta cells in islet of Langerhans.
Reactivity	Bovine, Human, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide with sequence from the C Terminus of Human SOCS4 (NP_543143.1, NP_955453.1).
Specificity	This antibody detects SOCS-4 at C-term.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	suppressor of cytokine signaling 4
Database Link	Entrez Gene 122809 Human UniProt ID Q8WXH5
Background	Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling) (1-3). Currently, there are eight members of the SOCS family that have been recognized, SOCS-1, -2, -3, -4, - 5, -6, -7 and CIS. Structurally, the SOCS proteins are composed of an N-terminus region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminus motif that has been called the SOCS box (4). The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STATinduced transcriptional mechanism (5). Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. Within the SOCS subfamily, SOCS-4, SOCS-5, SOCS-6, SOCS-7 remain poorly understood (6). On the basis of structural considerations, it would be expected that SOCS-4, -5, -6, and -7, like SOCS-1, SOCS-2, SOCS-3 and CIS, act to negatively regulate signal transduction (4), but it is uncertain which specific cytokine is involved (7-9).
Synonyms	Suppressor of cytokine signaling 4, SOCS-4, SOCS7, Suppressor of cytokine signaling 7, SOCS-7
Reference Data

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