AF9 (MLLT3) (C-term) Rabbit Polyclonal Antibody
CAT#: AP13157PU-N
AF9 (MLLT3) (C-term) rabbit polyclonal antibody, Purified
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CNY 6,314.00
货期*
5周
规格
Cited in 2 publications. |
Specifications
Product Data | |
Applications | IHC, WB |
Recommend Dilution | ELISA: 1/1,000 (peptide only) Western Blot: 1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/100. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | KLH conjugated synthetic peptide between 471-502 amino acid from the C-terminal region of Human MLLT3. |
Specificity | This antibody reacts to AF9 (MLLT3). |
Formulation | PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide |
Concentration | lot specific |
Purification | Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Predicted Protein Size | 63351 Da |
Gene Name | myeloid/lymphoid or mixed-lineage leukemia; translocated to, 3 |
Database Link | |
Background | The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed. |
Synonyms | YEATS3, Protein AF-9 |
Reference Data |
Citations (2)
The use of this Antibodies has been cited in the following citations: |
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The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures
,Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey TN, Meyer B, Varagnolo L, M|ller AM, Karas M, Dingermann T, Marschalek R,
Leukemia
,PubMed ID 21030982
[MLLT3]
|
The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling
,Bitoun E, Oliver PL, Davies KE,
Hum. Mol. Genet.
,PubMed ID 17135274
[MLLT3]
|
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