Osteocalcin (BGLAP) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/1000. Immunohistochemistry: 1/50-1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide KLH conjugated between 2~32 amino acids from the N-terminal region of Human Osteocalcin.
Specificity	This antibody detects Osteocalcin at N-term.
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	bone gamma-carboxyglutamate protein
Database Link	Entrez Gene 632 Human UniProt ID P02818
Background	Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2% of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.
Synonyms	Bone Gla protein, BGLAP
Note	Calculated Molecular weight: 10962 Da
Reference Data

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