LIN28B Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, WB
Recommend Dilution	Western blot: 1/1000.  Flow Cytometry: 1/10-1/50.  Immunofluorescence: 1/10-1/50. Immunohistochemistry on Paraffin Sections: 1/10-1/50.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of Human LIN28B.
Specificity	This antibody detects LIN28B at center.
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Purified by Protein A column followed by peptide affinity purifcation.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	26953 Da
Gene Name	lin-28 homolog B
Database Link	Entrez Gene 389421 Human UniProt ID Q6ZN17
Background	Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
Synonyms	LIN28B, CSDD2, Lin-28 homolog B
Reference Data

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