Plakophilin 2 (PKP2) (C-term) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunofluorescence Microscopy on Frozen Tissue and Cultured Cells (after Formaldehyde fixation). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (after Microwave retrieval treatment). For better accessibility of the desmosome-associated antigen Frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of nuclear PP2-staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended. Working Dilutions: 1/100 for Immunofluorescence Microscopy. Incubation Time: 1 h at RT, extended with paraffin sections.
Reactivity	Bovine, Human, Mouse, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Human synthetic Plakophilin 2 (C-terminal aa 820-837)
Specificity	This PP2 antiserum stains specifically desmosomal plaques present in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, Plakophilin 2 is highly enriched in the karyoplasm. Polypeptide Reacting: Plakophilin 2, Mr ~100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38) Tested Reactivity on Cultured Cell Lines: MCF-7, PLC.
Formulation	State: Serum State: Liquid stabilized antiserum with 0.09% Sodium Azide and 0.5% BSA
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	plakophilin 2
Database Link	Entrez Gene 5318 Human UniProt ID Q99959
Background	Plakophilin 2 is a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and in nonepithelial desmosome-possessing tissues such as myocardium, Purkinje fibers and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm.
Synonyms	PKP2
Reference Data

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