Cytokeratin 16 (KRT16) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence. Immunohistochemistry on Frozen and Paraffin Sections: 1/100-1/200. Incubation Time: 1h at RT. Positive Control: Rete ridges of Human foot sole epidermis, psoriatic skin.
Reactivity	Human, Mouse
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide of Human keratin K16 (formerly also designated cytokeratin 16; C-KE QSS SSF SQG QSS), coupled to KLH
Specificity	MW 48,000 (pI 5.1) intermediate filament polypeptide, keratin K16, detected by immunohistochemistry in psoriatic skin, in the rete ridges of foot sole epidermis, in tongue, esophagus, exocervix and in squamous cell carcinoma of skin, tongue and cervix. In the hair follicle, staining was restricted to the outer root sheath. Completely non-reactive were cells of non-stratified epithelia (e.g. liver, colon).
Formulation	State: Serum State: Liquid Serum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 16
Database Link	Entrez Gene 16666 MouseEntrez Gene 3868 Human UniProt ID P08779
Background	Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating. Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomas
Synonyms	Cytokeratin-16, Keratin-16, Keratin 16, KRT16, KRT16A, CK16, K16
Reference Data

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