MET Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on Paraffin Embedded Tissue: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around amino acids 1001~1005 (V-D-Y-R-A) derived from Human Met.
Specificity	The antibody detects endogenous levels of total Met protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MET proto-oncogene, receptor tyrosine kinase
Database Link	Entrez Gene 17295 MouseEntrez Gene 24553 RatEntrez Gene 4233 Human UniProt ID P08581
Background	The c-Met oncogene was originally isolated from chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a trans-membrane receptor-like protein with tyrosine kinase activity that is expressed in many tissues. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
Synonyms	Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met
Note	Molecular Weight: 156 kDa
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.