BRCA1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Immunofluorescence: 1/100 - 1/200. Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524 (Y-P-SP-Q-E)
Specificity	BRCA1 Antibody detects endogenous levels of total BRCA1protein.
Formulation	Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	BRCA1, DNA repair associated
Database Link	Entrez Gene 672 Human UniProt ID P38398
Background	BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.
Synonyms	BRCAI; BRCC1; BROVCA1; IRIS; PSCP; RNF53
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Ubiquitin mediated proteolysis

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