Biotin Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/3,000. Western Blot: 1/500 - 1/2,500. Immunohistochemistry: 1/200 - 1/1,000.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Specificity	This antibody reacts to Biotin.
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: AP State: Liquid Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	AP
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Background	Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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