Biotin Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	Immunofluorescence: 1/500 - 1/2,500. Flow Cytometry: 1/2,000 - 1/10,000.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Specificity	This antibody reacts to Biotin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: Rhodamine State: Lyophilized Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 5.0 moles TRITC per mole of Goat IgG
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Rhodamine
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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