Biotin Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC
Recommend Dilution	Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: Immunofluorescence: 1/500-1/2,500. Flow Cytometry:1/2,000-1/10,000. Note: All assays should be optimized by the user.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
Specificity	This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.
Formulation	0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4 Label: FITC State: Lyophilized IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Thimerisol Absorption emission: 495 nm / 528 nm Molar radio: 2.7 moles Fluorescein (FITC) per mole of IgG
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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