IL13 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/11,000. Western Blot: 1.5 - 2 µg/ml.
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein
Specificity	This antibody reacts to Interleukin-13.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide State: Aff - Purified State: Lyophilized
Reconstitution Method	Restore with 100 µL of deionized water or equivalent.
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q95J68
Background	Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ra) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.
Synonyms	IL-13, NC30
Reference Data

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