Biotin Ligase Tag / BLT Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/5,000. Western blot: 1/5,000.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	GLNDIFEAQKIEWH (Minimal BirA substrate; Biotin is added to the sequence at the Lysine)
Specificity	This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli Biotin ligase tag (BLT of BirA). This peptide is also called the Biotin Acceptor Peptide (BAP) of the Biotin Carboxyl Carrier Protein (BCCP) subunit of acetyl CoA carboxylase of E. coli. Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown). The peptide is a substrate for Biotin ligase, which places a Biotin moiety on the central lysine of the peptide when it interacts with BirA (the Biotin ligase). When proteins contain this sequence tag, they can be very efficiently pulled down after biotinylation using streptavidin beads.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Serum State: Liquid (sterile filtered) Serum Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	In Escherichia coli, transfer of biotin to the epsilon amino group of a specific lysine residue of the biotin carboxyl carrier protein (BCCP) is accomplished by the biotin holoenzyme synthetase BirA. The minimal substrate requirement of BirA-catalyzed biotinylation is a 14-residue peptide known as the biotin ligase tag (BLT). This antibody can be used to detect BLT in recombinant expression systems.
Synonyms	Biotin Ligase Epitoe Tag, BirA, bioR, dhbB
Reference Data

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