NOS1 (1422-1433) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Immunoprecipitation. Western Blot.
Reactivity	Human, Rat, Bat, Canine, Equine, Monkey, Mouse, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human nNOS amino acids 1422-1433
Specificity	This antibody is specific to nNOS amino acids 1422-1433.
Formulation	TBS, pH 7.4, containing 50% glycerol and 0.02% sodium azide State: Aff - Purified State: Liquid purified Ig fraction
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nitric oxide synthase 1
Database Link	Entrez Gene 4842 Human UniProt ID P29475
Background	Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).
Synonyms	bNOS, nNOS, NOS type I, Neuronal NOS, Constitutive NOS, NC-NOS
Reference Data

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