Human Lambda Light Chain Rabbit Polyclonal Antibody
CAT#: AP08133PU-S
Human Lambda Light Chain rabbit polyclonal antibody, Purified
Size: 1 ml
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CNY 3,740.00
货期*
5周
规格
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Specifications
Product Data | |
Applications | IF, IHC |
Recommend Dilution | Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/200 using streptavidin~biotin system or polymer system. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes). Recommended positive control: Human tonsil. |
Reactivity | Human, Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Purified human Lambda light chain from human Bence Jones proteins. |
Specificity | This antibody is useful in identification of leukemias, plasmasacytomas and certain non-Hodgkin's lymphomas. In normal human serum lambda chains are present 0.4-4.2 mg/ml. In multiple myeloma or B-cell malignancies these levels are increased. Higher amounts are also present in Bence Jones patient's urine. Cellular Localization: Cytoplasmic. |
Formulation | PBS, pH 7.4 containing 0.05% Sodium Azide as preservative and 1% BSA as stabilizer. State: Purified State: Liquid purified Ig fraction. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Background | Human Lambda chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA, IgG, IgM, IgD and IgE )and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30; most of them are bound to heavy chains of immunoglobulin. |
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