DYKDDDDK Epitope Tag Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot (1/500-1/1000).
Reactivity	Mammalian
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced by immunizing Rabbit with synthesized peptide containing the Flag epitope (D-Y-K-D-D-D-D-K).
Specificity	Flag-Tag Polyclonal Antibody AP08111PU detects Flag-tagged proteins overexpressed in cells. The antibody recognizes the Flag-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Purification	Immunoaffinity Chromatography using epitope-specific peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Synonyms	FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.