Myosin Light Chain 2 (MYL2) pSer18 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around phosphorylation site of serine 19 (A-T-Sp-N-V) derived from Human Myosin Light Chain 2.
Specificity	This antibody detects endogenous levels of Myosin Light Chain 2 only when phosphorylated at Serine 19.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% Glycerol Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	18 kDa
Gene Name	myosin light chain 2
Database Link	Entrez Gene 4633 Human UniProt ID P10916
Background	Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
Synonyms	MLC-2v, MLC-2
Reference Data

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