p53 (TP53) pSer6 Rabbit Polyclonal Antibody

CAT#: AP08001PU-N

p53 (TP53) pSer6 rabbit polyclonal antibody, Aff - Purified



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CNY 2,250.00


货期*
2周

规格
    • 100 ul

Product images

Specifications

Product Data
Applications IHC, WB
Recommend Dilution Western blot: 1/500~1/1000.
Immunohistochemistry on Paraffin Sections: 1/50~1/100.
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Phosphopeptide derived from Human p53 around the phosphorylation site of Serine 6 (P-Q-Sp-D-P).
Specificity This antibody detects endogenous levels of p53 only when phosphorylated at Serine 6.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Immunoaffinity Chromatography using epitope-specific peptide: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name tumor protein p53
Background p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Synonyms Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Note Molecuar Weight: 53 kDa
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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