Nestin (NES) (1484-1500) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/20000 - 1/80000. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 1/500 - 1/3000.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin
Specificity	This antibody recognizes Nestin.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nestin
Database Link	Entrez Gene 10763 Human UniProt ID P48681
Background	Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Synonyms	NES, Nbla00170
Reference Data

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