ZAP70 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/500-1/1000.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 250-300 of Human ZAP70.
Specificity	This antibody detects endogenous levels of ZAP-70 protein. (region surrounding Thr286)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 70 kDa
Gene Name	zeta chain of T cell receptor associated protein kinase 70kDa
Database Link	Entrez Gene 7535 Human UniProt ID P43403
Background	The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and ε chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodi-mer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A 70 kDa tyrosine phosphoprotein, ZAP-70, has been identified that associates with ζ and undergoes tyrosine phosphorylation following TCR stimulation.
Synonyms	ZAP-70, ZAP 70, SRK
Reference Data

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