S6K1 (RPS6KB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 380-430 of Human p70 S6K.
Specificity	This antibody detects endogenous levels of p70 S6K protein. (region surrounding Pro412)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% glycerol Preservative: 0.09% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~59,70,85 kDa
Gene Name	ribosomal protein S6 kinase B1
Database Link	Entrez Gene 72508 MouseEntrez Gene 83840 RatEntrez Gene 6198 Human UniProt ID P23443
Background	p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal. Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade. The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains.
Synonyms	Ribosomal protein S6 kinase I, S6K1, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, Serine/threonine-protein kinase 14A
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway

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