SHP1 (PTPN6) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 500-550 of Human SHP-1.
Specificity	This antibody detects endogenous levels of PTPN6 / SHP-1 protein (region surrounding Lys530).
Formulation	Phosphate buffered saline (PBS), approx. pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 15 mM Sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen (purity > 95% by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 68 kDa
Gene Name	protein tyrosine phosphatase, non-receptor type 6
Database Link	Entrez Gene 5777 Human UniProt ID P29350
Background	SHP (also designated short heterodimer partner and small heterodimer partner) is an orphan nuclear receptor containing the dimerization and ligandbinding domains found in other nuclear receptors but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues including fetal liver and adrenal gland, adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARγ. SHP interacts with nuclear receptors including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function via competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NF-κB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NF-κB. Lastly, negative feedback regulation of a hepatic bile acid transporter NTCP is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions.
Synonyms	PTPN-6, HCP, PTP1C, Protein-tyrosine phosphatase 1C, PTP-1C, SH-PTP1, Protein-tyrosine phosphatase SHP-1
Reference Data

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