p15 INK4b (CDKN2B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 100-150 of Human p15 INK4b.
Specificity	This antibody detects endogenous levels of p15 INK protein. (region surrounding Gly113)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure SDS-PAGE). Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 15 kDa
Gene Name	cyclin-dependent kinase inhibitor 2B
Database Link	Entrez Gene 12579 MouseEntrez Gene 25164 RatEntrez Gene 1030 Human UniProt ID P42772
Background	The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFβ-treated human keratinocytes, suggesting that p15 may act as an effector of TGFβ-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFβ-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.
Synonyms	p14INK4b, MTS2, p14-INK4b, p15-INK4b
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway

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