GJA1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunofluorescence: 1/50-1/200. Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 340-390 of Human Connexin 43.
Specificity	This antibody detects endogenous levels of GJA1 / Cx43 protein (region surrounding Ser369).
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze thaw cycles!
Predicted Protein Size	~43 kDa
Gene Name	gap junction protein alpha 1
Database Link	Entrez Gene 2697 Human UniProt ID P17302
Background	Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.
Synonyms	Gap junction alpha-1 protein, Connexin-43, GJAL
Reference Data

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