Caspase 5 (CASP5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western blot: 1.0 µg/ml - 2.0 µg/ml; detects a band of approximately 48 kDa in Ramos cell lysates. (Predicted precursor MWT 47.8kDa). Immuncytochemistry.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 14 amino acid peptide from the centre of human Caspase-5.
Specificity	This antibody recognises an epitope located within the internal region of human Caspase-5, a 47kDa cysteine protease, otherwise known as ICH-3.
Formulation	Phosphate buffered saline containing 0.02% Sodium Azide State: Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	caspase 5
Database Link	Entrez Gene 838 Human UniProt ID P51878
Background	Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta. The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).
Synonyms	CASP-5, CASP5, ICH3, ICH-3 protease, TY protease, ICE(rel)-III
Reference Data

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