ORAI3 (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections: 10 µg/ml. Western blot: 1 - 2 µg/ml; detects a band of approximately 35kDa in A20 cell lysates. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 15 amino acid peptide from near the amino terminus of human ORAI3.
Specificity	This antibody recognises human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family. This antibody is not expected to cross react with either ORAI1 or ORAI2.
Formulation	Phosphate buffered saline containing 0.02% Sodium Azide (NaN3) State: Purified State: Liquid purified IgG
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. 18 months from date of despatch.
Gene Name	ORAI calcium release-activated calcium modulator 3
Database Link	Entrez Gene 93129 Human UniProt ID Q9BRQ5
Background	Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.
Synonyms	TMEM142C, ORAI-3
Reference Data

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