RAD9A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1:2000 - 1:4000. Western Blot: 1:50 - 1:250; detects a band of approximately 60kDa.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).
Specificity	This antibody detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. This antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.
Formulation	Phosphate buffered saline pH7.2 containing 0.09% Sodium Azide (NaN3) State: Purified State: Liquid purified IgG
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	RAD9 checkpoint clamp component A
Database Link	UniProt ID Q99638
Background	In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.
Synonyms	RAD-9A, Cell cycle checkpoint control protein RAD9A, EC=3.1.11.2, DNA repair exonuclease rad9 homolog A
Reference Data

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