Hemoglobin Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA. Western Blot: 1/100-1/1000.
Reactivity	Bovine, Human
Host	Sheep
Clonality	Polyclonal
Immunogen	Native Hemoglobin from Erythrocytes
Specificity	This antibody is specific for Human Hemoglobin. It shows weak (< 10%) cross-reactivity with Bovine Hemoglobin.
Formulation	PBS containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Hemoglobin is a 66kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction. Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions. Mutations in the genes for the hemoglobin protein result in a group of hereditary diseases in humans termed hemoglobinopathies, and include sickle-cell disease and thalassemias.
Synonyms	Haemoglobin
Reference Data

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