Collagen I (COL1A1) (+ type III) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC
Recommend Dilution	ELISA: 1/500-1/2000. Immunofluorescence: 1/10-1/40. Immunohistochemistry on Frozen Sections: 1/10-1/40.
Reactivity	Human, Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Porcine Collagen, types 1 and 3 from skin
Specificity	This antibody reacts with native and heat denatured Porcine Collagen type 1 and 3. Less than 0.5% reactivity is observed with Porcine Albumin and Immunoglobulins.
Formulation	Mannitol, dextran and salts. State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore the vial (50 µg) with 500 µl of sterile distilled water or saline.
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	collagen type I alpha 1
Database Link	Entrez Gene 1277 Human UniProt ID P02452
Background	Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.