Complement C4A (C4A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunohistochemistry on frozen sections: 1/20 - 1/80. Immunohistochemistry on paraffin sections: 1/20 - 1/80; Heat induced epitope retrieval is recommended. Immunofluorescence: 1/20 - 1/80.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.
Specificity	This antibody is specific for complement split product C4d.
Formulation	Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 4A (Rodgers blood group)
Database Link	Entrez Gene 720 Human UniProt ID P0C0L4
Background	C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
Synonyms	Complement Component 4, CPAMD2, CPAMD3
Reference Data
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

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