VAV2 (SHD Region) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IP, WB
Recommend Dilution	Western Blot: 1:5,000. Immunoprecipitation.
Reactivity	Human
Host	Sheep
Clonality	Polyclonal
Immunogen	A his tagged fusion protein Vav-2 corresponding to amino acid sequence
Specificity	This antibody reacts to Vav-2 SHD.
Formulation	Phosphate buffered saline with 0.08% sodium azide State: Purified State: Liquid purified Ig (0.2 µm sterile filtered)
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	vav guanine nucleotide exchange factor 2
Database Link	Entrez Gene 7410 Human UniProt ID P52735
Background	The immunogen used for the anti-human Vav-2 SHD region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 578 - 878 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl- homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.
Synonyms	VAV-2
Reference Data

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