Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Immunofluoresence: 1/100 - 1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Specificity	This antibody detects endogenous levels of total Histone H2A.X protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Gene Name	H2A histone family member X
Database Link	Entrez Gene 3014 Human UniProt ID P16104
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities.
Synonyms	H2a/x, H2AFX, H2AX
Reference Data

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